世界*實驗材料供應商 Dianova 正式授權上海起發為(wei) 其中國代理， Dianova 在一直是行業(ye) 的*,一直為(wei) 廣大科研客戶提供zui為(wei) 的產(chan) 品和服務,上海起發一直秉承為(wei) 中國科研客戶帶來的產(chan) 品，的服務，簽約 Dianova 就是為(wei) 了給廣大科研客戶帶來更加完善的產(chan) 品和服務,您的滿意將是我們(men) zui大的收獲

 Dianova 中國代理, Dianova 上海代理， Dianova 北京代理，Dianova 廣東(dong) 代理， Dianova 江蘇代理Dianova 湖北代理,Dianova 天津,Dianova 黑龍江代理,Dianova 內(nei) 蒙古代理,Dianova 吉林代理,Dianova 福建代理, Dianova 江蘇代理, Dianova 浙江代理, Dianova 四川代理,

Dianova公司為(wei) 德國的病理免疫組化產(chan) 品供應商，具備的抗體(ti) 製備工藝及嚴(yan) 格的質控係統，產(chan) 品在上享有良好的聲譽。

克隆號為(wei) H09的IDH1（isocitrate dehydrogenase 1, 異檸檬酸鹽脫氫酶1）鼠單克隆抗體(ti) 為(wei) Dianova公司近期推出的產(chan) 品之一，此抗體(ti) 可特異性的檢測腦組織腫瘤中IDH1基因的R132H點突變。IDH1基因的第132密碼子的雜合突變是II 和III級神經膠質瘤（根據WHO的腫瘤學分類）的頻發事件。在大約70％的星形膠質細胞瘤和少突神經膠質細胞瘤可發現IDH1基因的R132H點突變。

IDH1基因R132H突變在部分腦腫瘤中的頻發現象使克隆號為(wei) H09的IDH1抗體(ti) 可高靈敏性和高特異性的用於(yu) 此類腫瘤的鑒別診斷中：如從(cong) 原發性膠質母細胞瘤中區分出間變性星形細胞瘤、從(cong) 毛細胞型星形細胞瘤或室管膜（細胞）瘤中區分出彌漫性星形細胞瘤（WHO分類II級），也可以用於(yu) 具有IDH1突變的腫瘤細胞浸潤前沿的與(yu) 反應性神經膠質增多症的區分。因此，由於(yu) 其高靈敏性和高特異性，此抗體(ti) 在腦部腫瘤的分類分級中以及在檢測單一或少量浸潤腫瘤細胞時是非常有用的。

DIA-303 – Anti-CD3 e (Ms) aus Ratte (Klon: HH3E) für Paraffingewebe aus Maus – 500 µl

Art.-Nr.                        DIA-303

Spezifität                      CD3 e

Spezies-Reaktivität             Maus

Wirtsspezies                     Ratte

Isotyp                           IgG1/k

Klon                             HH3E

Klonalität (Mono-/Polyklonal)     monoklonal

Anwendung                        Immunhistochemie (IHC)

                                 Immunhistochemie (Paraffingewebe)
                                 Gefrierschnitte (IHC-F)
                                 Western Blot

Konjugation                       unkonjugiert

Verdünnung                        Histo-/Zytochemie 1:100

Format                            Lyophilisat

                                  gereinigter Antikörper (aus Kulturüberstand)

Produktlinie / Thema               CD-Marker

                                   Immunhistochemie

Zweckbestimmung                     nur für Forschungszwecke

Suchcode                             DIA303M

                                     DIA303

Hersler / Marke                   dianova

Reactivity:

Antibody clone HH3E has been validated specifically for the detection of murine CD3 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). It detects a conserved epitope on the CD3 epsilon chain in a broad variety of species. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.
Cluster of differentiation 3 (CD3) is composed of four distinct polypeptide chains CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta, that form a multimeric protein complex. The CD3 complex associates non-covalently with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal trans-duction molecules. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into in-tracellular signals that elicit a change in the cell from a dormant to an activated state. During T cell maturation the expression of CD3 migrates from the cytoplasm of pro-thymocytes to the cell-membrane of thymocytes. The specific appearance at all stages of T cell development make CD3 an ideal marker for normal T cells and T cell neoplasms (lymphomas, leukemias). Moreover, CD3 is a usefull immunohistochemical marker for T cells in tissue sections.
In a clinical setting in humans, CD3 serves as an important T cell marker for the classification of malignant lymphomas and leu-kemias. It can also be used to detect T cells in coeliac disease, lymphocytic and collagenous colitis. An anti-CD3 epsilon anti-body (Okt3) has been clinically approved for the induction of immunosuppression in organ transplantation. In animal studies anti-CD3 antibodies can induce tolerance to allografts.

Immunohistochemistry of mouse CD3e (TCRE) in formalin-fixed paraffin-embedded tissue sections

The monoclonal antibody clone HH3E specifically stains mouse tissue sections by IHC-FFPE: Lymph nodes (A,B), spleen (C) and kidney (D).

All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer and counterstaining with Haematoxylin.

A

B

C

D

Related References:

1. Leon F. Flow cytometry of intestinal intraepithelial lymphocytes in celiac disease. Journal of Immunological Methods 363: 177-186, 2011
2. Smith-Garvin JE et al.. T cell activation. Annu Rev Immunol. 27:591-619, 2009
3. Sapp H et al.The terminal ileum is affected in patients with lymphocytic or collagenous colitis. Am J Surg Pathol. 26(11):1484-1492, 2002
4. Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clon-ality by polymerase chain reaction. Vet Immunol Immunopathol. 69:145-164, 1999
5. Mosnier et al. Lymphocytic and collagenous colitis: an immunohistochemical study. Am J Gastroenterol. 91(4):709-713, 1996
6. Chetty R, Gatter K. CD3: Structure, function, and role of immunostaining in clinical practice. The Journal of Pathology 173(4): 303–307, 1994
7. Salvadori S et al. Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J Immunol. 153(11):5176-5182, 1994
8. Nicolls MR et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation 55(3):459-468, 1993

相關(guan) 產(chan) 品信息如下：

我們(men) 公司zui大優(you) 勢是強大的采購，

1：基本什麽(me) 都能進口，血清，抗體(ti) ，耗材，還有部分限製進口的，

2：貨品全，現經營過700多個(ge) 品牌，基本所有生物試劑耗材都可以進口，特別是冷偏的產(chan) 品那就更有優(you) 勢，

3：提供加急服務，一般1-2周到貨，超過時限加急費全免

4：價(jia) 格公道，絕大部分價(jia) 格有優(you) 勢，當然不能保證100%產(chan) 品都是,因為(wei) 意味著沒有服務.

5：良好的信譽，大部分客戶我們(men) 提供貨到付款服務，客戶包括清華，北大 交大 複旦，中山等100多所大學，ROCHE，阿斯利康，國藥 ，fisher等500多家公司

6：我們(men) 還是Santa,Advanced Biotechnologies Inc, Dianova,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. Dako,Inc, GePromegaridege,Dianova  Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,Dianova , Polysciences,prospecbi, QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權代理。
7：我們(men) 還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發，歡迎合作。