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Dianova公司為(wei) 德國的病理免疫組化產(chan) 品供應商，具備的抗體(ti) 製備工藝及嚴(yan) 格的質控係統，產(chan) 品在上享有良好的聲譽。

克隆號為(wei) H09的IDH1（isocitrate dehydrogenase 1, 異檸檬酸鹽脫氫酶1）鼠單克隆抗體(ti) 為(wei) Dianova公司近期推出的產(chan) 品之一，此抗體(ti) 可特異性的檢測腦組織腫瘤中IDH1基因的R132H點突變。IDH1基因的第132密碼子的雜合突變是II 和III級神經膠質瘤（根據WHO的腫瘤學分類）的頻發事件。在大約70％的星形膠質細胞瘤和少突神經膠質細胞瘤可發現IDH1基因的R132H點突變。

IDH1基因R132H突變在部分腦腫瘤中的頻發現象使克隆號為(wei) H09的IDH1抗體(ti) 可高靈敏性和高特異性的用於(yu) 此類腫瘤的鑒別診斷中：如從(cong) 原發性膠質母細胞瘤中區分出間變性星形細胞瘤、從(cong) 毛細胞型星形細胞瘤或室管膜（細胞）瘤中區分出彌漫性星形細胞瘤（WHO分類II級），也可以用於(yu) 具有IDH1突變的腫瘤細胞浸潤前沿的與(yu) 反應性神經膠質增多症的區分。因此，由於(yu) 其高靈敏性和高特異性，此抗體(ti) 在腦部腫瘤的分類分級中以及在檢測單一或少量浸潤腫瘤細胞時是非常有用的。

DIA-404 – Anti-CD4 (Ms) from Rat (Clone: GHH4) for mouse FFPE tissue – 200 µl

Art.-Nr.                            DIA-404

Specificity                         CD4 (T4, MCH II co-receptor, T-helper cells)

Species Reactivity                  Mouse

Host Species                         Rat

Isotype                              IgG2b

Clone                                 GHH4

Clonality (Mono-/Polyclonal)          monoclonal

Application                       Immunohistochemistry (Paraffin-embedded Sections)

Conjugation                           unconjugated

Format                                lyophilisate

                                      antibody purified (from culture supernatant)

Product line / Topic                   CD markers

                                       immunohistochemistry

Intended Use                            for Research Use Only

Search Code                             DIA404

Manufacturer / Brand                    dianova

Reactivity

Antibody clone GHH4 has been developed and validated for the detection of murine CD4 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH4 identifies T helper cells in murine FFPE tissue by IHC.
CD4 is a marker for T helper cells. Besides this subpopulation of mature T cells (helper/inducer), CD4 is also expressed on most thymocytes and weakly on macrophages and dendritic cells.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two different co-receptors: CD8 and CD4. CD4 is a nonpolymorphous 55 kD glycoprotein belonging to the immunoglobulin superfamily. It acts as a co-receptor with the TCR during antigen-induced T cell activation and thymic differentiation by binding MHC class II and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail.
In humans, CD4 is expressed in the majority of T-cell lymphomas. CD4 plays an important role in the classification of lymphocytes in inflammatory lesions and malignant lymphomas. CD4 serves as HIV receptor on T cells, macrophages and brain and is downregulated by HIV proteins during AIDS progression.

IHC for CD4 in mouse FFPE tissue

Mouse spleen: The monoclonal antibody clone GHH4 specifically stains CD4 in standard FFPE mouse spleen. Incubation with antibody clone GHH4 (10μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with ImmPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Chris Bonnas, Synaptic Systems, Göttingen, Germany).

General References:

1. Foti M et al. p56Lck anchors CD4 to distinct microdomains on microvilli. Proc Natl Acad Sci U S A. 2002, 99: 2008-13.
2. Brady et al. Crystal structure of domains 3 and 4 of rat CD4: relation to the NH2-terminal domains. Science 1993, 260:979-83
3. Janeway CA et al. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992, 10: 645-74.
4. Bierer BE et al. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989, 7: 579-99.
5. Fleischer B et al. Function of the CD4 and CD8 molecules on human T lymphocytes: regulation of T cell triggering. Jour-nal of Immunology 1996, 136: 1625-8
6. Wilde DB et al. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lym-phocyte lines. J Immunol. 1983, 131: 2178-83.
7. Dialynas et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal anti-body GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983, 131: 2445-51.

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