世界*實驗材料供應商 Dianova 正式授權上海起發為(wei) 其中國代理， Dianova 在一直是行業(ye) 的*,一直為(wei) 廣大科研客戶提供zui為(wei) 的產(chan) 品和服務,上海起發一直秉承為(wei) 中國科研客戶帶來的產(chan) 品，的服務，簽約 Dianova 就是為(wei) 了給廣大科研客戶帶來更加完善的產(chan) 品和服務,您的滿意將是我們(men) zui大的收獲

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Dianova公司為(wei) 德國的病理免疫組化產(chan) 品供應商，具備的抗體(ti) 製備工藝及嚴(yan) 格的質控係統，產(chan) 品在上享有良好的聲譽。

克隆號為(wei) H09的IDH1（isocitrate dehydrogenase 1, 異檸檬酸鹽脫氫酶1）鼠單克隆抗體(ti) 為(wei) Dianova公司近期推出的產(chan) 品之一，此抗體(ti) 可特異性的檢測腦組織腫瘤中IDH1基因的R132H點突變。IDH1基因的第132密碼子的雜合突變是II 和III級神經膠質瘤（根據WHO的腫瘤學分類）的頻發事件。在大約70％的星形膠質細胞瘤和少突神經膠質細胞瘤可發現IDH1基因的R132H點突變。

IDH1基因R132H突變在部分腦腫瘤中的頻發現象使克隆號為(wei) H09的IDH1抗體(ti) 可高靈敏性和高特異性的用於(yu) 此類腫瘤的鑒別診斷中：如從(cong) 原發性膠質母細胞瘤中區分出間變性星形細胞瘤、從(cong) 毛細胞型星形細胞瘤或室管膜（細胞）瘤中區分出彌漫性星形細胞瘤（WHO分類II級），也可以用於(yu) 具有IDH1突變的腫瘤細胞浸潤前沿的與(yu) 反應性神經膠質增多症的區分。因此，由於(yu) 其高靈敏性和高特異性，此抗體(ti) 在腦部腫瘤的分類分級中以及在檢測單一或少量浸潤腫瘤細胞時是非常有用的。

DIA-808 – Anti-CD8a (Ms) from Rat (Clone: GHH8) for mouse FFPE tissue – 200 µl

Art.-Nr.                          DIA-808

Specificity                       CD8a (MHC I Co-Receptor, cytotox. T-cells)

Species Reactivity                Mouse

Host Species                       Rat

Isotype                            IgG2a/k

Clone                              GHH8

Clonality (Mono-/Polyclonal)       monoclonal

Application                       Immunohistochemistry (Paraffin-embedded Sections)

Conjugation                        unconjugated

Format                            lyophilisate

                                  antibody purified (from culture supernatant)

Product line / Topic               CD markers

                                   immunohistochemistry

Intended Use                        for Research Use Only

Search Code                         DIA808

Manufacturer / Brand                dianova

Reactivity:

Antibody clone GHH8 has been developed and validated for the detection of murine CD8a in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH8 identifies cytotoxic/suppressor T-cells in murine FFPE tissue by IHC.
CD8 is a marker for T-cells with suppressor and cytotoxic activity. CD8 is also found on the surface of some natural killer cells, most thymocytes, some T-cell lymphomas and large granular lymphocyte leukemias.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two dif-ferent co-receptors: CD8 and CD4. CD8 was first discovered as a cell surface marker in mice and was used to discriminate CD8+ CTLs and CD4+ Th cells. CD8a is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). CD8 alpha beta is expressed on the majority of cytotoxic T-lymphocytes (CTLs). This CD8 heter-omeric proteins (with approx. 32-34 KDa each monomer) are physically associated with a p56 tyrosine kinase to mediate T-cell activation signals through phosphorylation of adjacent proteins.
Besides its important role in T-cell signalling, the CD8 co-receptor is also important for CD8+ T-cell development, stabilisation of the interface between CD8+ T-cells and target cells, and activation of intestinal epithelial T lymphocytes (iIELs). Therefore, CD8 has been the target of many therapeutic strategies designed to modulate T-cell responses during auto-immune reactions, allergy and organ transplant rejection.

Immunohistochemistry of mouse CD8a in formalin-fixed paraffin-embedded tissue sections

A, Mouse spleen: The monoclonal antibody clone GHH8 specifically stains CD8a in standard formalin-fixed paraffin-embedded mouse spleen. Incubation with antibody clone GHH8 (5μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with Im-mPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Chris Bonnas, Synaptic Systems, Göttingen, Germany).
B, Mouse liver after viral infection: Specific Detection of invading T-cells with monoclonal antibody clone GHH8 by IHC-FFPE. Note the clear membrane-bound signal free of background (left picture). Detection with a Ventana automated stainer following standard procedures. Incubation for 30min at 4°C with 10μg/ml of antibody clone GHH8, corresponding to a 1:50 dilution (Pic-ture courtesy of Susanne Krasemann, Mouse Pathology Core Facility, UKE, Hamburg, Germany).

A

B

References

1. Kersh AE et al. Cutting Edge: Resident Memory CD8 T Cells Express High-Affinity TCRs. Journal of Immunology 2015, 195: 3520-4.
2. Wu RC et al. New insights on the role of CD8(+)CD57(+) T-cells in cancer. Oncoimmunology 2012, 1: 954-56.
3. Hyland L et al. Mice lacking CD8+ T cells develop greater numbers of IgA-producing cells in response to a respiratory vi-rus infection. Virology 1994, 204: 234-41.
4. Prince HE et al. CD4 and CD8 subsets defined by dual-color cytofluorometry which distinguish symptomatic from asymp-tomatic blood donors seropositive for human immunodeficiency virus. Diagnostic & Clinical Immunology 1987, 5: 188-93.
5. Gaudernack G et al. Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies di-rectly conjugated to monosized magnetic microspheres. Journal of Immunological Methods 1986, 90: 179-87.
6. Fleischer B et al. Function of the CD4 and CD8 molecules on human cytotoxic T lymphocytes: regulation of T cell trigger-ing. Journal of Immunology 1986,136: 1625-8.

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