genelink 40-5131-05說明書

genelink 40-5131-05說明書(shu)

產(chan) 品目錄號 40-5131-05

產(chan) 品名稱 LiCl RNA 沉澱溶液 (7.5M LiCl，50 mM EDTA pH 8.0)；50 mL

Size50 mL

DescriptionLiCl RNA 沉澱溶液 (7.5M LiCl，50 mM EDTA pH 8.0)；50 mLDo 不用於(yu) 小於(yu) 300 個(ge) 核苷酸的 RNA 沉澱。終濃度為(wei) 2.5M 的 LiCl 在不添加乙醇的情況下可有效沉澱大於(yu) 300 個(ge) 核苷酸的 RNA，這種沉澱方法選擇性沉澱 RNA，不能有效沉澱 、蛋白質或碳水化合物。它是從(cong) RNA 製劑中去除翻譯或 c 合成抑製劑的優(you) 選方法。它還提供了一種從(cong) 體(ti) 外轉錄反應中回收 RNA 的簡單快速方法。在 4 °C 下以 12K rpm 離心 20 min，可定量回收低至 50 ng 的 RNA。

用途：2.5 M 氯化鋰終濃度，不添加乙醇或異丙醇。

濃度≥100 ng/mL 時 rna 的常規沉澱。

Catalog Number

40-5131-05

Product Name

LiCl RNA Precipitation Solution (7.5M LiCl, 50 mM EDTA pH 8.0); 50 mL

Size

50 mL

Description

LiCl RNA Precipitation Solution (7.5M LiCl, 50 mM EDTA pH 8.0); 50 mL

Do not use for precipitation of RNA smaller than 300 nucleotides.

A final concentration of 2.5M LiCl is effective in precipitation of RNA larger than 300 nucleotides without the addition of

ethanol, this method of precipitation selectively precipitates RNA and does not efficiently precipitate , protein or

carbohydrate. It is the method of choice for removing inhibitors of translation or c synthesis from RNA preparations. It

also provides a simple rapid method for recovering RNA from in vitro transcription reactions. As little as 50 ng of RNA can be

quantitatively recovered by centrifugation at 12K rpm for 20 minutes at 4°C.

Usage: 2.5M Lithium Chloride final concentration without addition of ethanol or isopropanol. Routine precipitation of

RNA at Concentrations ≥100 ng/mL.